Ethics in Archaeological Lidar

Airborne laser scanning or lidar has now been used by archaeologists for twenty years, with many of the first applications relying on data acquired by public agencies seeking to establish baseline elevation maps, mainly in Europe and North America. More recently, several wide-area acquisitions have been designed and commissioned by archaeologists, the most extensive of which cover tropical forest environments in the Americas and Southeast Asia. In these regions, the ability of lidar to map microtopographic relief and reveal anthropogenic traces on the Earth’s surface, even beneath dense vegetation, has been welcomed by many as a transformational breakthrough in our field of research. Nevertheless, applications of the method have attracted a measure of criticism and controversy, and the impact and significance of lidar are still debated. Now that wide-area, high-density laser scanning is becoming a standard part of many archaeologists’ toolkits, it is an opportune moment to reflect on its position in contemporary archaeological practice and to move towards a code of ethics that is vital for scientific research. The papers in this Special Collection draw on experiences with using lidar in archaeological research programs, not only to highlight the new insights that derive from it but also to cast a critical eye on past practices and to assess what challenges and opportunities remain for developing codes of ethics. Using examples from a range of countries and environments, contributions revolve around three key themes: data management and access; the role of stakeholders; and public education. We draw on our collective experiences to propose a range of improvements in how we collect, use, and share lidar data, and we argue that as lidar acquisitions mature we are well positioned to produce ethical, impactful, and reproducible research using the technique

“This is wrong and we will support you”: The (restricted) role of resident-led neighbourhood-level planning teams in residential displacement

Using qualitative data collected from 2013-2017, this paper investigates how resident-led planning teams working within Hamilton, Ontario’s Neighbourhood Action Strategy (NAS) responded to gentrification and displacement. Highlighting case studies of three neighbourhoods, our findings reveal that resident groups can act as both sites of support for gentrification and sites of resistance to residential displacement. Our findings complicate the common narrative that posits residents as powerless in the face of gentrification, showing how residents resisted coercive displacementefforts. The case studies expose concrete strategies used by residents: engaging directly with City Hall and developers, countering exclusionary neighbourhood attitudes through community dialogue, and supporting tenant organizing. However, findings also highlight how differences among residents (particularly class, race, and length and type of tenure) shaped both the nature and effectiveness of resistance in this municipally-supported initiative, and identify the need for more attention to preventing displacement within formal planning processes.S’appuyant sur les données d’une évaluation quinquennale de la Stratégie d’action de quartier (NAS) de Hamilton, en Ontario, le présent document explore le rôle que les équipes de quartier peuvent jouer pour lutter contre les déplacements résidentiels. La NAS, agissant à l’échelle municipale et composée d’intervenants multiples, a été mise sur pied pour répondre aux inégalités en santé qui se retrouvent au niveau des quartiers ; elle soutient les « équipes de planification » dirigées par les résidents pour développer des communautés plus saines. À l’aide de données qualitatives recueillies de 2013 à 2017, cet article examine les interventions des équipes de planification dirigées par les résidents envers l’embourgeoisement et les déplacements. En mettant en lumière des études de cas de trois quartiers, nos résultats révèlent que les groupes de résidents peuvent être tout autant des foyers d’appui à l’embourgeoisement que des foyers de résistance aux déplacements résidentiels. Nos résultats compliquent le récit commun qui postule que les résidents sont impuissants face à l’embourgeoisement, en montrant comment les résidents sont intervenus et sont devenus de puissants acteurs pour résister aux efforts de déplacement forcé. Les études de cas divulguent des stratégies concrètes utilisées par les résidents : dialoguer directement avec les élus et le personnel de l’hôtel de ville, de même qu’avec les promoteurs ; contrecarrer les attitudes d’exclusion des résidents du quartier par le biais de dialogues communautaires ; et appuyer les locataires dans leurs efforts d’organisation

Enhanced differentiation of retinal progenitor cells using microfabricated topographical cues

Due to the retina’s inability to replace photoreceptors lost during retinal degeneration, significant interest has been placed in methods to implant replacement cells. Polymer scaffolds are increasingly being studied as vehicles for cellular delivery to degenerated retinas. Previously, we fabricated poly(methyl methacrylate) thin film scaffolds that increased survival and integration of implanted retinal progenitor cells (RPCs). Additionally, these scaffolds minimized the trauma and cellular response associated with implantation of foreign bodies into mouse eyes. Here, we demonstrate that biodegradable polycaprolactone (PCL) thin film scaffolds can be fabricated with integrated microtopography. Microfabricated topography in a PCL thin film enhanced the attachment and organization of RPCs compared to unstructured surfaces. Using real-time quantitative polymerase chain reaction we also observed that attachment to microtopography induced cellular differentiation. RPCs grown on PCL thin films exhibited an increase in gene expression for the photoreceptor markers recoverin and rhodopsin, an increase in the glial and Müller cell marker GFAP, and a decrease in SOX2 gene expression (a marker for undifferentiated progenitor cells) compared to cells grown on unmodified tissue culture polystyrene (TCPS)

Retinal tissue engineering using mouse retinal progenitor cells and a novel biodegradable, thin-film poly(e-caprolactone) nanowire scaffold

Retinal progenitor cells (RPCs) can be combined with nanostructured polymer scaffolds to generate composite grafts in culture. One strategy for repair of diseased retinal tissue involves implantation of composite grafts of this type in the subretinal space. In the present study, mouse retinal progenitor cells (RPCs) were cultured on laminin-coated novel nanowire poly(e-caprolactone)(PCL) scaffolds, and the survival, differentiation, and migration of these cells into the retina of C57bl/6 and rhodospsin −/− mouse retinal explants and transplant recipients were analyzed. RPCs were cultured on smooth PCL and both short (2.5 μm) and long (27 μm) nanowire PCL scaffolds. Scaffolds with adherent mRPCs were then either co-cultured with, or transplanted to, wild-type and rhodopsin −/− mouse retina. Robust RPC proliferation on each type of PCL scaffold was observed. Immunohistochemistry revealed that RPCs cultured on nanowire scaffolds increased expression of mature bipolar and photoreceptor markers. Reverse transcription polymerase chain reaction revealed down-regulation of several early progenitor markers. PCL-delivered RPCs migrated into the retina of both wild-type and rhodopsin knockout mice. The results provide evidence that RPCs proliferate and express mature retinal proteins in response to interactions with nanowire scaffolds. These composite grafts allow for the migration and differentiation of new cells into normal and degenerated retina

The evolution of agro-urbanism: A case study from Angkor, Cambodia

16 pages. Published in "Journal of Anthropological Archaeology" by Elsevier: A.K. Carter, S. Klassen, M.T. Stark, M. Polkinghorne, P. Heng, D.H. Evans, R. Chhay; The evolution of agro-urbanism: a case study from Angkor, Cambodia; J. Anthropol. Archaeol., 63 (2021), Article 101323The vast agro-urban settlements that developed in the humid tropics of Mesoamerica and Asia contained both elite civic-ceremonial spaces and sprawling metropolitan areas. Recent studies have suggested that both local autonomy and elite policies facilitated the development of these settlements; however, studies have been limited by a lack of detail in considering how, when, and why these factors contributed to the evolution of these sites. In this paper, we use a fine-grained diachronic analysis of Angkor’s landscape to identify both the state-level policies and infrastructure and bottom-up organization that spurred the growth of Angkor as the world’s most extensive pre-industrial settlement complex. This degree of diachronic detail is unique for the ancient world. We observe that Angkor’s low-density metropolitan area and higher-density civic-ceremonial center grew at different rates and independently of one another. While local historical factors contributed to these developments, we argue that future comparative studies might identify similar patterns.The Authors thank the APSARA National Authority for their permission to conduct remote sensing and field investigations. Thanks also to Roland Fletcher for his support. Thank you to Terry and Eileen Lustig for their comments and suggestions on aspects of economic geography and Michael E. Smith for reading and offering comments on an earlier draft of this paper. Thanks to Malay So and Maryne Dana for administrative support. Research, planning, and writing of this manuscript was undertaken with the support of the University of Oregon Global Oregon Faculty Collaboration Fund supported by the Global Studies Institute in the UO Office of International Affairs. Data collection for parts of the research in this study have been funded by: the National Geographic Society Committee for Research and Exploration under Grant 9602-14; Dumbarton Oaks under a Project Grant in Garden and Landscape Studies; the Social Sciences and Humanities Research Council of Canada Postdoctoral Fellowship; the National Science Foundation Doctoral Dissertation Research Improvement Awards (#1638137); Australian Research Council Discovery Early Career Researcher Award (DE150100756) and Council Discovery Grant (DP170102574); and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreements 639828 and 866454)

Multi-dose Oral Ondansetron for Pediatric Gastroenteritis: study Protocol for the multi-DOSE oral ondansetron for pediatric Acute GastroEnteritis (DOSE-AGE) pragmatic randomized controlled trial

BACKGROUND: There are limited treatment options that clinicians can provide to children presenting to emergency departments with vomiting secondary to acute gastroenteritis. Based on evidence of effectiveness and safety, clinicians now routinely administer ondansetron in the emergency department to promote oral rehydration therapy success. However, clinicians are also increasingly providing multiple doses of ondansetron for home use, creating unquantified cost and health system resource use implications without any evidence to support this expanding practice. METHODS/DESIGN: DOSE-AGE is a randomized, placebo-controlled, double-blinded, six-center, pragmatic clinical trial being conducted in six Canadian pediatric emergency departments (EDs). In September 2019 the study began recruiting children aged 6 months to 18 years with a minimum of three episodes of vomiting in the 24 h preceding enrollment,(1:1 allocation via an internet-based, third-party, randomization service) to receive a 48-h supply (i.e., six doses) of ondansetron oral solution or placebo, administered on an as-needed basis. All participants, caregivers and outcome assessors will be blinded to group assignment. Outcome data will be collected by surveys administered to caregivers 24, 48 and 168 h following enrollment. The primary outcome is the development of moderate-to-severe gastroenteritis in the 7 days following the ED visit as measured by a validated clinical score (the Modified Vesikari Scale). Secondary outcomes include duration and frequency of vomiting and diarrhea, proportions of children experiencing unscheduled health care visits and intravenous rehydration, caregiver satisfaction with treatment and safety. A preplanned economic evaluation will be conducted alongside the trial. DISCUSSION: Definitive data are lacking to guide the clinical use of post-ED visit multidose ondansetron in children with acute gastroenteritis. Usage is increasing, despite the absence of supportive evidence. The incumbent additional costs associated with use, and potential side effects such as diarrhea and repeat visits, create an urgent need to evaluate the effect and safety of multiple doses of ondansetron in children focusing on post-emergency department visit and patient-centered outcomes. TRIAL REGISTRATION: ClinicalTrials.gov: NCT03851835. Registered on 22 February 2019

A pragmatic randomized controlled trial of multi-dose oral ondansetron for pediatric gastroenteritis (the DOSE-AGE study): statistical analysis plan.

BACKGROUND: Acute gastroenteritis is a leading cause of emergency department visits and hospitalizations among children in North America. Oral-rehydration therapy is recommended for children with mild-to-moderate dehydration, but children who present with vomiting are frequently offered intravenous rehydration in the emergency department (ED). Recent studies have demonstrated that the anti-emetic ondansetron can reduce vomiting, intravenous rehydration, and hospitalization when administered in the ED to children with dehydration. However, there is little evidence of additional benefit from prescribing ondansetron beyond the initial ED dose. Moreover, repeat dosing may increase the frequency of diarrhea. Despite the lack of evidence and potential adverse side effects, many physicians across North America provide multiple doses of ondansetron to be taken following ED disposition. Thus, the Multi-Dose Oral Ondansetron for Pediatric Gastroenteritis (DOSE-AGE) trial will evaluate the effectiveness of prescribing multiple doses of ondansetron to treat acute gastroenteritis-associated vomiting. This article specifies the statistical analysis plan (SAP) for the DOSE-AGE trial and was submitted before the outcomes of the study were available for analysis. METHODS/DESIGN: The DOSE-AGE study is a phase III, 6-center, placebo-controlled, double-blind, parallel design randomized controlled trial designed to determine whether participants who are prescribed multiple doses of oral ondansetron to administer, as needed, following their ED visit have a lower incidence of experiencing moderate-to-severe gastroenteritis, as measured by the Modified Vesikari Scale score, compared with a placebo. To assess safety, the DOSE-AGE trial will investigate the frequency and maximum number of diarrheal episodes following ED disposition, and the occurrence of palpitations, pre-syncope/syncope, chest pain, arrhythmias, and serious adverse events. For the secondary outcomes, the DOSE-AGE trial will investigate the individual elements of the Modified Vesikari Scale score and caregiver satisfaction with the therapy. DISCUSSION: The DOSE-AGE trial will provide evidence on the effectiveness of multiple doses of oral ondansetron, taken as needed, following an initial ED dose in children with acute gastroenteritis-associated vomiting. The data from the DOSE-AGE trial will be analyzed using this SAP. This will reduce the risk of producing data-driven results and bias in our reported outcomes. The DOSE-AGE study was registered on ClinicalTrials.gov on February 22, 2019. TRIAL REGISTRATION: ClinicalTrials.gov NCT03851835 . Registered on 22 February 2019

The Development, Deployment, and Evaluation of the CLEFT-Q Computerized Adaptive Test:A Multimethods Approach Contributing to Personalized, Person-Centered Health Assessments in Plastic Surgery

BackgroundRoutine use of patient-reported outcome measures (PROMs) and computerized adaptive tests (CATs) may improve care in a range of surgical conditions. However, most available CATs are neither condition-specific nor coproduced with patients and lack clinically relevant score interpretation. Recently, a PROM called the CLEFT-Q has been developed for use in the treatment of cleft lip or palate (CL/P), but the assessment burden may be limiting its uptake into clinical practice. ObjectiveWe aimed to develop a CAT for the CLEFT-Q, which could facilitate the uptake of the CLEFT-Q PROM internationally. We aimed to conduct this work with a novel patient-centered approach and make source code available as an open-source framework for CAT development in other surgical conditions. MethodsCATs were developed with the Rasch measurement theory, using full-length CLEFT-Q responses collected during the CLEFT-Q field test (this included 2434 patients across 12 countries). These algorithms were validated in Monte Carlo simulations involving full-length CLEFT-Q responses collected from 536 patients. In these simulations, the CAT algorithms approximated full-length CLEFT-Q scores iteratively, using progressively fewer items from the full-length PROM. Agreement between full-length CLEFT-Q score and CAT score at different assessment lengths was measured using the Pearson correlation coefficient, root-mean-square error (RMSE), and 95% limits of agreement. CAT settings, including the number of items to be included in the final assessments, were determined in a multistakeholder workshop that included patients and health care professionals. A user interface was developed for the platform, and it was prospectively piloted in the United Kingdom and the Netherlands. Interviews were conducted with 6 patients and 4 clinicians to explore end-user experience. ResultsThe length of all 8 CLEFT-Q scales in the International Consortium for Health Outcomes Measurement (ICHOM) Standard Set combined was reduced from 76 to 59 items, and at this length, CAT assessments reproduced full-length CLEFT-Q scores accurately (with correlations between full-length CLEFT-Q score and CAT score exceeding 0.97, and the RMSE ranging from 2 to 5 out of 100). Workshop stakeholders considered this the optimal balance between accuracy and assessment burden. The platform was perceived to improve clinical communication and facilitate shared decision-making. ConclusionsOur platform is likely to facilitate routine CLEFT-Q uptake, and this may have a positive impact on clinical care. Our free source code enables other researchers to rapidly and economically reproduce this work for other PROMs

Novel Antioxidant Properties of Doxycycline

Doxycycline (DOX), a derivative of tetracycline, is a broad-spectrum antibiotic that exhibits a number of therapeutic activities in addition to its antibacterial properties. For example, DOX has been used in the management of a number of diseases characterized by chronic inflammation. One potential mechanism by which DOX inhibits the progression of these diseases is by reducing oxidative stress, thereby inhibiting subsequent lipid peroxidation and inflammatory responses. Herein, we tested the hypothesis that DOX directly scavenges reactive oxygen species (ROS) and inhibits the formation of redox-mediated malondialdehyde-acetaldehyde (MAA) protein adducts. Using a cell-free system, we demonstrated that DOX scavenged reactive oxygen species (ROS) produced during the formation of MAA-adducts and inhibits the formation of MAA-protein adducts. To determine whether DOX scavenges specific ROS, we examined the ability of DOX to directly scavenge superoxide and hydrogen peroxide. Using electron paramagnetic resonance (EPR) spectroscopy, we found that DOX directly scavenged superoxide, but not hydrogen peroxide. Additionally, we found that DOX inhibits MAA-induced activation of Nrf2, a redox-sensitive transcription factor. Together, these findings demonstrate the under-recognized direct antioxidant property of DOX that may help to explain its therapeutic potential in the treatment of conditions characterized by chronic inflammation and increased oxidative stress